Volume 34, Issue 1NovemberPages Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities Author links open overlay panel Y. Young Show more https: The use of phosphate solubilizing bacteria as inoculants increases the P uptake by plants. In this study, isolation, screening and characterization of 36 strains of phosphate solubilizing bacteria PSB from Central Taiwan were carried out.
Isolation of Phosphate Solubilizing Bacteria Bacterial isolates were isolated from soil samples by using root-free soil as nonrhizosphere and also by using soil which is surrounding the root of oil palm as Rhizosphere. Bacteria which represent clearing halozone on the plates were selected and purified on PVK media [ 27 ].
Read "Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea, Soil Biology and Biochemistry" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. ii ABSTRACT Nitrogen and phosphorus are essential macronutrients for plant growth. The major sources of N and P in agriculture are chemical fertilizers. ISOLATION AND CHARACTERIZATION OF PHOSPHATE SOLUBLIZING BACTERIA FROM ANAND AGRICULTURE SOIL. Isolation of phosphate solubilizing Bacteria from soil sample Preparation of media for isolation of PSM Analysis Software (version ) and quality value.
The eight bacterial isolates were selected for further study based on the high performance of phosphate solubilization [ 28 ]. The concentration and purity of the extracted DNA were determined spectrophotometrically [ 29 ]. Two universal oligonucleotide forward and reverse primers were synthesized based on the standard 16S rRNA gene sequence [ 30 ].
The primers used to amplify the 16S rDNA samples were forward primer: The tubes were subjected to 35 cycles in a thermal cycler with the following program: The gel was stained with ethidium bromide and photographed using gel-documentation system Hoefer PS XT [ 29 ].
The 16S rRNA gene products of eight bacteria isolates were sequenced.
The clean PCR product was subjected to cycle sequencings in both directions using the universal primers. The phylogenetical analysis was conducted using the Neighbor Joining Method. Then, the output trees were performed with the software Molecular Evolutionary Genetics Analysis version 4.
Results and Discussion 3. The primer amplified the gene successfully from all of the phosphate solubilizing bacterial isolates, although there were no obvious variations in the size of rRNA gene products between the eight bacterial isolates.
The size of the 16S rRNA gene product of all isolated bacteria in this study was about 1. The 16S rRNA gene sequence of phosphates solubilizing bacteria that was isolated from oil palm soil was compared with Genbank and received the accession numbers Table 2.
The 16S rRNA gene sequences allowed separation between isolates at the species level.rock phosphate (NPRP) and biofertilizer (phosphate solubilizing bacteria/PSB) on soil pH, pHо (zero point of charge, ZPC) and organic-C in one subgroup of Andisols, namely Acrudoxic Durudands, Ciater Region West Java.
solublizing bacteria, pesticide, soil, Bacillus sp., Pseudomonas sp.
A. Isolation of Phosphate Solubilizing Bacteria (PSB) A total of 50 Phosphate solublizing bacterial (PSB) colonies were isolated from repeatedly chemical pesticide used well as quantitative analysis of phosphate solubilizing activity of the selected isolates were.
phosphate-solubilizing bacteria (PSB) and arbuscular inoculated with AMF, the result of which was an mycorrhizal fungi (AMF).increase concentration of both phosphorus and Application of biological fertilizers such as biological nitrogen in the plant biomass . soil inoculation with phosphate-solubilizing bacteria is known to improve solubilization of ﬁxed soil phosphorus and applied phosphates, resulting in higher crop yields.
ISOLATION AND CHARACTERIZATION OF PHOSPHATE SOLUBLIZING BACTERIA FROM ANAND AGRICULTURE SOIL. Isolation of phosphate solubilizing Bacteria from soil sample Preparation of media for isolation of PSM Analysis Software (version ) and quality value.
These results supported by that application of PGPR along with phosphate-solubilizing bacteria is highly effective for improving yield in Maize crop.
|Characterization of phosphate-solubilizing bacteria isolated from calcareous soils - ScienceDirect||A soil survey was conducted at Doda district in the month of April to assess the various physico-chemical and biological properties of the soil. Composite surface soil samples were collected at a depth of cm.|
|Phylogenetic Relationship of Phosphate Solubilizing Bacteria according to 16S rRNA Genes||This study aimed to evaluate the capacity of PGPB, identified by phenotypic and 16S rRNA sequencing from a vegetable purple soil in Chongqing, China, to increase soil nitrogen Nphosphorus Pand potassium K availability and growth of kiwifruit Actinidia chinensis. In doing so, three out of 17 bacterial isolates with a high capacity of N2-fixation Bacillus amyloliquefaciens, XD-N-3P-solubilization B.|
|Introduction||The total consumption of US Biofertilizer Market was ,|
PGPR treatment increased germination rate and root/shoot growth in way similar to indole acetic acid (IAA), cytokinin and gibberellins treatments [ 11 ].