The compound microscope uses lenses and light to enlarge the image and is also called an optical or light microscope versus an electron microscope. The simplest optical microscope is the magnifying glass and is good to about ten times 10x magnification. The compound microscope has two systems of lenses for greater magnification, 1 the ocular, or eyepiece lens that one looks into and 2 the objective lens, or the lens closest to the object.
In this type of microscope, there are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen. Although sometimes found as monocular with one ocular lens, the compound binocular microscope is more commonly used today. The first light microscope dates back towhen Zacharias Jansen created a compound microscope that used collapsing tubes and produced magnifications up to 9X.
Because it contains its own light source in its base, a compound light microscope is also considered a bright field microscope.
Bright field microscopy simply means that the specimen is lit from below and viewed from above. Magnification In order to ascertain the total magnification when viewing an image with a compound light microscope, take the power of the objective lens which is at 4x, 10x or 40x and multiply it by the power of the eyepiece which is typically 10x.
Therefore, a 10x eyepiece used with a 40X objective lens, will produce a magnification of X. The naked eye can now view the specimen at a magnification times greater and so microscopic details are revealed.
More on objective lenses here. Magnification is the ability to view an object as larger. A good image is obtained when the amount of specimen detail is also increased. Magnification alone will not achieve this.
Good resolution or the resolving power of the microscope is necessary to see the valuable details comprised in an image. Resolving Power is the ability to measure the separation of images that are close together.
Optical quality plays a vital role but the distance of the wavelength of light used is crucial. With a shorter wavelength, you have increased resolution.
Working Distance At low magnification your working distance is longer and so vice versa when increasing magnification. Damage to your specimen is inevitable if you are not cautious of the shorter working distance when increasing your magnification. Be especially careful with oil immersion lenses.
This objective has the smallest working distance and your careful handling is important. BrightField Microscopy What can be viewed: Using Stained Prepared slides you should see bacteria, chromosomes, organelles, protist or metazoans, smears, blood, negative stained bacteria and thick tissue sections.
Utilizing unstained wet mounts for living preparations should enable you to see pond water, living protists or metazoans, and plant cells such as algae. Distortion is a factor in viewing smaller specimens and the difficulty increases further without natural pigmentation to provide some contrast when viewing the specimen.How to Use a Compound Microscope.
In this Article: Article Summary Understanding Your Microscope Focusing the Microscope Community Q&A A compound microscope is a powerful magnification tool commonly used in scientific laboratories for viewing bacteria and other tiny cell samples. 2: a non-optical instrument (such as one using radiations other than light or using vibrations) for making enlarged images of minute objects an acoustic microscope.
The Motic microscope (room ) is very similar to the Hund microscope (room ) except that they are equipped with a phase contrast condenser. Microscope: Microscope, instrument that produces enlarged images of small objects, allowing the observer an exceedingly close view of minute structures at a scale convenient for examination and analysis.
It may provide a dynamic image (as with optical instruments) or one that is static (as with scanning electron microscopes).
A compound light microscope is a microscope with more than one lens and its own light source. In this type of microscope, there are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen.
Compound Light Microscope: The microscope pictured above is referred to as a compound light microscope. The term light refers to the method by which light transmits the image to your eye. Compound deals with the microscope having more than one regardbouddhiste.comcope is the combination of two words; "micro" meaning small and "scope" .